Effects of Nano Calcium Supplemented Milk on Bone Mineral Metabolism in Ovariectomized Rats

Postmenopausal osteoporosis is a series problem in elderly women and is characterized by decrease in bone mass, leading to fracture and imbalanced turnover of the bone. Overietomized rats exhibits a decrease in mineral density, volume and strength of bone, and an increase in bone turnover that was seen in women suffering from osteoporosis.

Milk is an excellent source for calcium, considering the absorption of calcium and its bioavailability. Calcium from calcium carbonate has also been reported as a good calcium source compared with calcium from milk, but some studies reported that calcium from milk is better than calcium carbonate. Presently, there are commercially available calcium-fortified milk products. However, the calcium availability of those is not well understood when those are added into milk.

Studies show that the intake of calcium has never been sufficient. Among various sources of dietary calcium, little information is available about nano-sized calcium. Therefore, study on the effect of bone metabolism with nano-calcium and other kinds of calcium-fortified milks was carried out on oveariectomized rats.

This study examined the effects of calcium supplemented milk on bone loss in ovariectomized rats. Twenty four Sprague-Dawley female rats, 7 weeks-old, were divided into 4 groups, All rats were fed with a ml of milk containing different type of calcium supplements.

After 18 weeks of feeding, body weight gain and food efficiency ratio were significantly different among them.


OVX1, no calcium supplement milk

OVX2, calcium carbonate supplemented milk

OVX3, ionized calcium supplemented milk

OVX4, nano-calcium supplemented milk

Observations as follow:

  • Serum concentration of calcium and phosphorus were not different among groups.
  • There was a significant difference in calcium content of femoral weight in OVX4 compared to other groups.
  • Femoral bone mineral density was significantly greater in OVX4 than the other groups (p<0.05).
  • The OVX4 group also showed highest stiffness, maximum energy in femur and trabecular bone area.

This study allowed us to define the effects of nano-calcium in lessening the decrease in bone mineral density and increase in bone resorption induced by ovariectomy. The difference in metaphyseal trabeculae was clearly found between nano-calcium supplemented and ovariectomized rats. The nano-calcium supplemented femur has elongated trabeculae whereas no calcium supplemented group displayed fragmented metaphyseal trabeculae. Observation indicated that nano-calcium may induce in high intestinal absorption and inhibit bone loss by ovariectomy.

As the conclusion of the study, NANO CALCIUM supplement milk is proved as the most effective for the improvement and prevention from OSTEOPOROSIS being induced from bone.


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* The study had been conducted by Department of Food Science and Technology, Sejong University, Korea, and it was supported by NanoTechWorld (Pohang, Korea).

Clinical Test Results on Osteoporosis, Spinal Stenosis Patients

Clinical tests have been carried by several hospitals on more than 50 patients, whom has either osteoporosis or osteopenia problem. The patients were given treatment by taking nano-calcium for several months. The result of the tests show that the conditions of the patients osteoporosis / osteopenia has been healed, and other symptoms caused by lack of calcium have also been cured.

The following clinical test report shows that the osteoporosis condition of a 59 years old osteoporosis patient has improved from T-score -3.86 to T-score -1.95.


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